Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

Figure 2

Bucillamine suppresses the catalytic activation of caspase-3 and caspase-8 in HEI-OC1 cells treated with cisplatin. Cells were pretreated with 2 mM bucillamine for 1 h, followed by the addition of 20 μM cisplatin for 24 h. Cells lysates were reacted with Ac-IETD-pNA and Ac-DEVD-pNA, the p-NA-conjugated specific substrates for caspase-3 (a) and caspase-8 (b), respectively. Enzymatic activity assays for caspases were performed according to colorimetric assay at 405 nm absorbance using a spectrophotometer. Data are shown as the mean (s.d.) of triplicate experiments. *P<0.01 compared with control.

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