Figure 3
Bucillamine suppresses cisplatin-induced caspase-3 activation in HEI-OC1 cells. (a) Cells were pretreated with 2 mM bucillamine for 1 h followed by the addition of 20 μM cisplatin for 24 h. Cells were then washed and fixed, and the active form of caspase-3 was determined by immunofluorescence. Pictures were taken using a fluorescence microscope (original magnification, × 600). (b) Equal amounts of total proteins (50 μg per lane) from cell lysate of the above experimental conditions were prepared, and subject to SDS-PAGE and western blot analyses.
