Figure 7
Bucillamine induces phase II antioxidants and detoxifying enzymes in HEI-OC1 cells treated with cisplatin. (a) Cells were pretreated with 2 mM bucillamine for 1 h followed by the addition of 20 μM cisplatin at 12 h. Phase II antioxidant genes (HO-1, SOD1 and SOD2) from total RNA were amplified using specific primer sets. Expression levels of HO-1, SOD1 and SOD2 were significantly increased by the pretreatment with bucillamine as compared with cisplatin alone at 12 h. (b) Cell lysates were electrophoresed through an SDS-polyacrylamide gel and subject to immunoblot analysis for HO-1, SOD1 and SOD2.
