Figure 8 | Experimental & Molecular Medicine

Figure 8

From: Bucillamine prevents cisplatin-induced ototoxicity through induction of glutathione and antioxidant genes

Figure 8

siRNA knockdown of HO-1 and SOD2 enzymes does not completely block the protective effects of bucillamine. (a) After transfection of HEI-OC1 cell with double-stranded siRNA (HO-1, SOD2 or both), cells were pretreated with various concentrations of bucillamine for 1 h followed by the addition of 20 μM cisplatin for 24 h. Then, cell viability was measured by MTT assays. (b) Cell lysates were subject to immunoblot analysis with antibodies against pro-caspase-3 and active form of caspase-3. β-actin was used as a control.

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