Figure 2
Overlap of target genes identified by the two high-throughput experiments. (a) Venn diagram showing concordant and discordant BAF53b target genes identified in two recent high-throughput studies. Of the 62 genes found to be upregulated in the microarray analysis, only 2 were also shown to be differentially expressed in the RNA-sequencing (RNA-seq) experiment. Among the downregulated genes identified by the microarray analysis, only Actl6b (BAF53b) was shared between the two data sets. When the P-value cutoff was loosened, only one and two genes were additionally shared among the up and downregulated genes, respectively. Colors and symbols: U, math symbol for union; red font, dysregulated after learning; black font, dysregulated at baseline; *, oppositely dysregulated in the two studies (P<0.20 and P⩾0.05). (b) Comparison of the two studies. The high-throughput platforms differed, as did the ages and genotypes of the utilized mice. Vogel-Ciernia et al.5 made six comparisons, whereas Wu et al.15 made only two. Here, we looked at genes that were dysregulated at baseline or after learning. HC, home cage; OLM, object location memory.
