Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Silencing of KIF14 interferes with cell cycle progression and cytokinesis by blocking the p27Kip1 ubiquitination pathway in hepatocellular carcinoma

Figure 3

Effects of kinesin family member 14 (KIF14) knockdown on expression of cyclins and cyclin-dependent kinase inhibitors. (a) Western blot analysis shows the downregulation of cyclins B1, D1 and E1 and the upregulation of p27Kip1 in the KIF14-specific short interfering RNA (siKIF14)-treated cells. There was no difference in the levels of p16Ink4a and p21Cip1 between the siKIF14- and siNEG-transfected cells. (b) Double immunochemical staining shows a negative correlation between KIF14 (green) and p27Kip1 (red). DAPI, 4′-6-diamidino-2-phenylindole.

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