Figure 5 | Experimental & Molecular Medicine

Figure 5

From: Enhanced proliferation and differentiation of Oct4- and Sox2-overexpressing human adipose tissue mesenchymal stem cells

Figure 5

Adipogenic differentiation assay using Oct4/Sox2-ATMSCs and RT–PCR analysis for adipogenic markers. (a) Oil red O staining for lipid droplets in Oct4/Sox2-transfected ATMSCs was strong at 7, 14 and 21 days during adipogenic differentiation compared with RFP-ATMSCs. (b) RT–PCR results show a significant increase in PPARγ and lipoprotein lipase mRNA expression at 7, 14 and 21 days during adipogenic differentiation. Band densities of PPARγ in RT–PCR were evaluated semi-quantitatively by densitometry. Results are the ratio of PPARγ expression normalized to GAPDH mRNA levels. Data are representative of three independent experiments with similar results. (1) RFP-ATMSCs, (2) Oct4/Sox2-ATMSCs.

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