Figure 6 | Experimental & Molecular Medicine

Figure 6

From: Enhanced proliferation and differentiation of Oct4- and Sox2-overexpressing human adipose tissue mesenchymal stem cells

Figure 6

Osteogenic differentiation assay using Oct4/Sox2-ATMSCs and RT–PCR analysis for osteogenic markers. (a) Alizarin red S staining for mineralization in Oct4/Sox2-ATMSCs was strong at 7, 14 and 21 days during osteogenic differentiation compared with RFP-ATMSCs. (b) RT–PCR results show upregulation of collagen I and osteocalcin mRNA levels in Oct4/Sox2-ATMSCs at 7, 14 and 21 days during osteogenic differentiation compared with RFP-ATMSCs. Band densities of collagen I and osteocalcin in RT–PCR were evaluated semi-quantitatively by densitometry. Results are the ratio of collagen I and osteocalcin expression normalized to GAPDH mRNA levels. Data are representative of three independent experiments with similar results. (1) RFP-ATMSCs, (2) Oct4/Sox2-ATMSCs.

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