Figure 5
From: CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation
Phosphorylation of Drp1S616 modifies its oligomerization and inhibits mitochondrial fragmentation in neurons. (a–e, a′′–e′) Morphology of mitochondria in cells expressing YFP (a, a′), YFP-hDrp1 (b, c, b′, c′) or its phosphorylation mutants, which are YFP-hDrp1(S616A) (d, d′) and YFP-hDrp1(S616D) (e, e′). These clones were cotransfected with DsRed-mito into cultured hippocampal neurons at DIV. Magnified images of straight mitochondrial morphology in white dotted boxes are shown at the bottom each panel. (b, c) Examples of neuron exhibiting punctate (b′) or filamentous (c′) form of YFP-hDrp1. (f) Proportion of neurons showing filamentous YFP-hDrp1. *P<0.05, n=5. (g) Proportion of neurons showing fragmented mitochondria. *P<0.05, n=5.
