Figure 3
Silencing growth hormone receptor (GHR) inhibited invasion and metastasis through epithelial to mesenchymal transition (EMT). (a) Matrigel invasion assay was performed in GHR transfected PANC-1 and HPAC. Cells that had invaded through matrigel gel-coated Boyden chamber were fixed and stained with crystal violet. (b) Cells that invaded were counted and expressed as percentage of invasion. (c) EMT markers were inhibited in GHR silenced PANC-1 and HPAC cells. Protein expression levels of Notch-2, Snail, E-cadherin, N-Cadherin, Zeb, Vimentin and Slug along with internal control β-actin were analyzed by western blotting in scrambled control and GHR silenced PANC-1 and HPAC cells. (d) Percentage expression of proteins was calculated using densitometric values obtained from density ratio of EMT markers/β-actin. PS-PANC-1 Scrambled, PG-PANC-1 GHR silenced, HS-HPAC Scrambled, HG-HPAC GHR silenced. Data presented as the mean±s.d. (n=3). *P<0.05.
