Figure 3
From: miR-27 regulates mitochondrial networks by directly targeting the mitochondrial fission factor
miR-27 inhibits the mitochondrial fission factor (MFF) mRNA translation. (a) Polysome profiles. Cell lysates, prepared from CHANG liver cells transfected with pre-miR-27, anti-miR-27 or control miRNA, were fractionated through sucrose gradients. The arrow indicates the direction of sedimentation; − indicates fractions with no ribosomal components; 40S and 60S indicate small and large ribosomal subunits, respectively; 80S indicates monosomes; and LMWP and HMWP indicate low- and high-molecular-weight polysomes, respectively. (b) The relative distributions of the MFF (top) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs (bottom) were studied by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis of each of the 13 fraction. The data are representative of two independent experiments.
