Figure 4
From: Inhibition of phospholipase D2 induces autophagy in colorectal cancer cells
PLD2 inhibition increases the on-rate of autophagy. (a) HT29 cells were co-transfected with RFP-LC3 and GFP-Lamp1 and then pre-treated with BafA1 (100 nM) or PLD2 inhibitor (10 μM) for 10 h, and the colocalization of RFP-LC3 and GFP-Lamp-1 was analyzed. Representative fluorescence microphotographs are shown together with the profiles of colocalization. (b) The cells were treated with PLD2 inhibitor and/or BafA1 for 10 h, and the lysates were immunoblotted with the indicated antibody. The data are representative of three independent experiments.
