Figure 1

Assessment of the clinical deterioration, radiographic bone destruction and pathologic abnormalities in collagen-induced arthritis (CIA). CIA was induced in male mSIRT1 KO (KO) and wild-type (WT) littermates at 14–18 weeks old. (a) The mean clinical scores and ankle thickness were determined on the indicated days after primary immunization. (b) The gross hindpaw appearance and plain ankle radiographs of representative mice at Day 39 are shown (left panel). Semiquantification of bone destruction with the radiographic scoring system (right panel). (c) Ankle joints in representative hematoxylin and eosin - and safranin-O-stained sections (× 100) (left panel) are shown, along with the mean pathologic scores for synovial inflammation and cartilage damage (right panel). The results of three independent experiments were pooled (total of 10 mice per group). (d) Arthritic joints in representative sections stained for F4/80 or LY6G (left panel). The mean macrophage and neutrophil counts (right panel) from four randomly selected high-power fields (× 400) in each ankle joint (n=5 mice for each group) are shown. The values are expressed as the means±s.e., *P<0.05, ^**P<0.01 vs WT.