Figure 4 | Experimental & Molecular Medicine

Figure 4

From: Myeloid deletion of SIRT1 suppresses collagen-induced arthritis in mice by modulating dendritic cell maturation

Figure 4

Effects of SIRT1 deletion on type II collagen (CII)-specific T-cell proliferation and cytokine production. The cells from the regional lymph nodes of the mSIRT1 KO (KO) and wild-type (WT) mice were isolated on Day 28 and incubated with vehicle (VEH) or CII (10 or 50 μg ml−l) for 72 h. (a) The proliferative response was assessed by the fluorescence of 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFSE). The dot plots (left panel) represent one of four experiments, and the graph (right panel) shows proportion of CFSE-positive cells (4 mice per group). (b) The IFN-γ and IL-17 levels in the culture supernatants were determined by enzyme-linked immunosorbent assay (7–10 mice per group). The values are expressed as the means±s.e., *P<0.05, **P<0.01 vs WT.

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