Figure 5

Effect of SIRT1 deletion on LPS-induced DC maturation. The bone marrow-derived DCs from the mSIRT1 KO (KO) and wild-type (WT) mice were treated with phosphate-buffered saline or LPS (50 ng ml^−l) for 24 h. (a) Western blot analysis of SIRT1 expression in the DCs before and after LPS stimulation. (b) The endocytic capacity of the DCs was determined by FITC-conjugated dextran uptake. The bar graph shows the amount of FITC-dextran (%) in the CD11c⁺ cells. (c) The expression of the surface molecules was determined by flow cytometry and staining with anti-CD80, anti-CD86, anti-MHC class I or anti-MHC class II antibodies in the CD11c⁺-gated cells. (d) Enzyme-linked immunosorbent assay was performed to determine the levels of IL-12p70, IL-1β, IL-6 and IL-10 in the LPS-treated DCs from the KO and WT mice. The values are expressed as the means±s.e. of three experiments. *P<0.05, ^**P<0.01 vs WT.