Table 1 Sequences and accession numbers for the primers (forward, FOR; reverse, REV) used in real-time RT–PCR
Gene | Primer sequences | Accession no. |
|---|---|---|
SIRT1 | FOR:GACACAGAGACGGCTGGAA REV: CAGACCCTCAAGCCATGTTT | NM_ 019812 |
TNF-α | FOR: GCTGTCCCTGCGCTTCA REV:CTCGTCCCCAATGACATCCT | NM_ 013693 |
IL-1β | FOR:CCATGGCACATTCTGTTCAAA REV:GCCCATGAGAGGCAAGGA | NM_ 008361 |
IL-2 | FOR:AACTCCCCAGGATGCTCAC REV:CGCAGAGGTCCAAGTTCATC | NM_008366 |
IL-4 | FOR:TCGGCATTTTGAACGAGGTC REV:GAAAAGCCCGAAAGAGTCTC | NM_021283 |
IL-6 | FOR: CCACGGCCTTCCCTACTTC REV: TTGGGAGTGGTATCCTCTGTGA | NM_ 031168 |
IL-13 | FOR: CGCAAGGCCCCCACTAC REV:AAAGTGGGCTACTTCGATTTTGG | NM_008355 |
IL-17 | FOR: TGAGCTTCCCAGATCACAGA REV: TCCAGAAGGCCCTCAGACTA | NM_ 010552 |
IL-23 | FOR: GCTCCCCTTTGAAGATGTCA REV: GACCCACAAGGACTCAAGGA | NM_ 031252 |
IL-10 | FOR: TGTCAAATTCATTCATGGCCT REV: ATCGATTTCTCCCCTGTGAA | NM _010548 |
IFN-γ | FOR: TGAGCTCATTGAATGCTTGG REV: ACAGCAAGGCGAAAAAGGAT | NM_ 008337 |
MCP-1 | FOR: TGTCACCCTTGGAGCTCATG REV: TTTTTCGACTTTTATCCTCTGTTG | NM_ 011333 |
MMP-3 | FOR: CCCCTGATGTCCTCGTGGTA REV: GCACATTGGTGATGTCTCAGGTT | NM_ 010809 |
MMP-13 | FOR: GGTCCTTGGAGTGATCCAGA REV: TGATGAAACCTGGACAAGCA | NM_ 008607 |
T-bet | FOR: TTCCCATTCCTGTCCTTCAC REV: CCACATCCACAAACATCCTG | NM_ 019507 |
ROR-γT | FOR: TGAGGCCATTCAGTATGTGG REV: CTTCCATTGCTCCTGCTTTC | NM_011281 |
GATA3 | FOR: GGAAACTCCGTCAGGGCTA REV: AGAGATCCGTGCAGCAGAG | NM_008091 |
Foxp3 | FOR: CCTGCCTTGGTACATTCGTG REV: TGTTGTGGGTGAGTGCTTTG | NM_054039 |
GAPDH | FOR: CGTCCCGTAGACAAAATGGT REV: TTGATGGCAACAATCTCCAC | NM_008084 |
