Figure 2 | Experimental & Molecular Medicine

Figure 2

From: Vaccination with Klebsiella pneumoniae-derived extracellular vesicles protects against bacteria-induced lethality via both humoral and cellular immunity

Figure 2

In vivo immunogenicity of K. pneumoniae EVs. (a) K. pneumoniae EV-reactive IgG antibody in serum from mice immunized with 10, 100, 100 ng of K. pneumoniae EVs. This antibody was measured by ELISA (n=5; *P<0.05, **P<0.01 and ***P<0.001, compared with PBS group). (b) E. coli EV-reactive and K. pneumoniae EV-reactive IgG antibody in serum from mice immunized with 0.1 and 1 μg of K. pneumoniae EVs and 1 μg of E. coli EVs (n=5; *P<0.05 and **P<0.01, compared with PBS group). (c) Secreted IFN-γ, IL-17 and IL-4 from splenocytes with and without stimulation with anti-CD3 and anti-CD28 antibodies. The mice were immunized with 10, 100, 1000 ng of K. pneumoniae EVs (n=5; *P<0.05 and **P<0.01, compared with the non-stimulated, PBS-injected group).

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