Figure 3

Proportion of IFN-γ⁺, IL-17⁺ and IL-4⁺ CD4⁺ T cells. The mice splenocytes, which were immunized with 10, 100, 1000 ng of K. pneumoniae EVs three times for 3 weeks at a 1-week interval, were stimulated with anti-CD3 and anti-CD28 antibodies. (a) The graphs and (b) FACS dot plots of intracellular cytokine staining assay for IFN-γ, IL-17 and IL-4 in splenic CD4+ T cells. CD3+, CD4+ and cytokine-positive splenocytes were gated using APC, FITC and PE, respectively (n=3; *P<0.05, **P<0.01 and ***P<0.001, compared with PBS group).