Figure 1
From: Aldehyde dehydrogenase is used by cancer cells for energy metabolism
High aldehyde dehydrogenase (ALDH) isoform expression levels are associated with poor overall survival in non-small-cell lung cancer (NSCLC). (a) Metabolic gene clustering of samples from lung adenocarcinoma patients from The Cancer Genome Atlas (TCGA). The clustered genes in set A were upregulated in the tumor group, and 150 metabolic genes with P<0.05 are listed. (b) Clustering analysis of six ALDH isoforms. Data for ALDH18A1 (P=1.31 × 10−38), ALDH1B1 (P=3.84 × 10−15), ALDH3B2 (P=1.17 × 10−10), ALDH1L2 (P=1.58 × 10−05), ALDH7A1 (P=1.09 × 10−4) and ALDH1L1 (P=2.32 × 10−2) are shown. Gene expression values were normalized using standard normalization (mean=0 and s.d.=1). Each row of the heat map is sorted in an ascending order according to the P-value. (c) Relative Aldefluor activity from various NSCLC cell lines and primary human lung cells. **P<0.01, ***P<0.001. (d) Immunohistochemical staining of ALDH isoforms in normal and cancerous lung tissue. Scale bar=100 μm. (e) Expression of ALDH3A1, ALDH3A2 and ALDH3B2 in cancerous (CA, triangles) and normal lung type I and II pneumocytes (NL, circles). *P<0.001, n=59 for each sample.
