Table 4 Expression constructs of ALDH isoforms used in this study

From: Aldehyde dehydrogenase is used by cancer cells for energy metabolism

		Insert
No	Vector	Gene template	Primer sequences		Enzyme site	Amplicon size (bp)
1	p3XFlag-CMV	ALDH1A1	F′	TGACGATGACAAGCTTATGTCATCCTCAGGCACG	HindIII	1506
			R′	TCGCGGCCGCAAGCTT TTATGAGTTCTTCTGAGAG	HindIII
2	p3XFlag-CMV	ALDH1A3	F′	TGACGATGACAAGCTT ATGGCCACCGCTAACGGGGC	HindIII	1539
			R′	TCGCGGCCGCAAGCTT TCAGGGGTTCTTGTCGCC	HindIII
3	p3XFlag-CMV	ALDH1B1	F′	TGACGATGACAAGCTT ATGCTGCGCTTCCTGGCAC	HindIII	1554
			R′	TCGCGGCCGCAAGCTT TTACGAGTTCTTCTGAGG	HindIII
4	p3XFlag-CMV	ALDH2	F′	TGACGATGACAAGCTT ATGTTGCGCGCTGCCGCC	HindIII	1554
			R′	TCGCGGCCGCAAGCTT TTATGAGTTCTTCTGAGGC	HindIII
5	p3XFlag-CMV	ALDH3A1	F′	TGACGATGACAAGCTT ATGAGCAAGATCAGCGAG	HindIII	1362
			R′	TCGCGGCCGCAAGCTT TCAGTGCTGGGTCATCTTG	HindIII
6	p3XFlag-CMV	ALDH3A2	F′	TGACGATGACAAGCTT ATGGAGCTCGAAGTCCGGC	HindIII	1527
			R′	TCGCGGCCGCAAGCTT TCATCTCTGCTTACTGGAC	HindIII
7	p3XFlag-CMV	ALDH3B1	F′	TGACGATGACAAGCTT ATGGACCCCCTTGGGGAC	HindIII	1407
			R′	TCGCGGCCGCAAGCTT TCAGAGCAGTGTGCAGCTG	HindIII
8	p3XFlag-CMV	ALDH4A1	F′	TGACGATGACAAGCTT ATGCTGCTGCCGGCGCCCG	HindIII	1692
			R′	TCGCGGCCGCAAGCTT TCACTGCATGTACGCGTAG	HindIII
9	p3XFlag-CMV	ALDH5A1	F′	TGACGATGACAAGCTT ATGGCGACCTGCATTTGGC	HindIII	1647
			R′	TCGCGGCCGCAAGCTT CTACAAGCCCCCGTAACAC	HindIII
10	p3XFlag-CMV	ALDH6A1	F′	TGACGATGACAAGCTT ATGGCGGCGCTATTGGCG	HindIII	1608
			R′	TCGCGGCCGCAAGCTT CTAACGGCCCATGGTAGGC	HindIII
11	p3XFlag-CMV	ALDH7A1	F′	TGACGATGACAAGCTT ATGTGGCGCCTTCCTCGC	HindIII	1620
			R′	TCGCGGCCGCAAGCTT TTACTGAAACTTGATTCC	HindIII
12	p3XFlag-CMV	ALDH9A1	F′	TGACGATGACAAGCTT ATGTTTCTCCGAGCAGGC	HindIII	1557
			R′	TCGCGGCCGCAAGCTT TCAAAAAGCAGATTCCACATC	HindIII
13	p3XFlag-CMV	ALDH1L1	F′	TGACGATGACAAGCTT ATGAAGATTGCAGTGATTG	HindIII	2709
			R′	TCGCGGCCGCAAGCTT TCAGTATTCGAAGGTCAC	HindIII
14	p3XFlag-CMV	ALDH1L2	F′	TGACGATGACAAGCTT ATGCTGCGGCGGGGCAGC	HindIII	2772
			R′	TCGCGGCCGCAAGCTT CTAATATTCCAGTGTCAC	HindIII
15	p3XFlag-CMV	ALDH18A1	F′	TGACGATGACAAGCTT ATGTTGAGTCAAGTTTAC	HindIII	2382
			R′	TCGCGGCCGCAAGCTT TCAGTTGGTGTTTCTCTG	HindIII

Abbreviations: ALDH, aldehyde dehydrogenase.

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Table 4 Expression constructs of ALDH isoforms used in this study

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