Figure 1
From: IL-17A exacerbates diabetic retinopathy by impairing Müller cell function via Act1 signaling
IL-17A and IL-17RA expression is upregulated in Akita mouse retina and HG-treated Müller cells. At 3 months after the onset of diabetes, the mice were killed, and the retinas were removed for analysis via western blot (a), ELISA (b) and fluorescent immunohistochemistry (c). Immunofluorescent staining on retinal cross-sections indicates that GS-labeled Müller cells cover approximately the entire retinal thickness, and they express IL-17A and IL-17RA in Akita mice, indicated by the arrows. The ganglion cells labeled by NeuN in the retinal GCL did not express IL-17A or IL-17RA. (d) Primarily cultured rat retinal Müller cells, which were labeled by GS, exceeded 98% in the cell purity. (e) IL-17A expression in primarily cultured Müller cells. (f) IL-17A concentration in Müller cell culture supernatants. **P<0.01 vs wild-type (WT) mice or control. ELISA, enzyme-linked immunosorbent assay; GCL, ganglion cell layer; GS, glutamine synthetase; HG, high glucose; IL, interleukin; INL, inner nuclear layer; ONL, outer nuclear layer.
