Figure 2
TFAP2C (transcription factor-activating enhancer-binding protein 2C)-induced TGFBR1 (transforming growth factor-β receptor type 1) upregulation promotes non-small-cell lung cancer (NSCLC) tumorigenesis. (a) The effects of TFAP2C or TGFBR1 knockdown on the proliferation of NCI-H292 cells were measured by a colony-forming assay. (b) The effects of TFAP2C or TGFBR1 overexpression on the proliferation of MRC5 cells were measured by a colony-forming assay. (c) The effects of TFAP2C or TGFBR1 knockdown on the viability of NCI-H292 cells were measured by a cell viability assay. *P<0.05 compared with control cells; **P<0.05 compared with cells treated with TFAP2C small interfering RNA (siRNA). (d) The effects of TFAP2C or TGFBR1 overexpression on the viability of MRC5 cells were measured by a cell viability assay. *P<0.05 compared with control cells; **P<0.05 compared with cells transfected with TFAP2C. (e) The effects of TFAP2C or TGFBR1 knockdown on cell cycle progression of NCI-H292 cells were determined by cell cycle analysis. (f) The effects of TFAP2C or TGFBR1 overexpression on cell cycle progression of MRC5 cells were determined by cell cycle analysis. Error bars represent±s.e.m. (n=3); one-way analysis of variance (ANOVA), Tukey’s honestly significant difference test.
