Figure 1 | Experimental & Molecular Medicine

Figure 1

From: In vitro generation of mature midbrain-type dopamine neurons by adjusting exogenous Nurr1 and Foxa2 expressions to their physiologic patterns

Figure 1

Exogenous expression patterns derived from retroviral and lentiviral vectors with various promoters in NPCs during differentiation in vitro. The reporter GFP gene was engineered into retroviral and lentiviral vectors with various universal promoters. NPCs derived from rat embryonic cortices at E14 were transduced with each of the GFP-expressing vectors, and the transduced NPCs were differentiated into neurons from the day following transduction. (a–c) GFP expression by various expression systems at differentiation day 3 (D3). Representative GFP+ cell images (a). Viral transduction efficiency was estimated by % GFP+ cells of total DAPI+ cells (b) and GFP fluorescence intensity (MFI) of individual cells (c). (d–g) Expression of exogenous GFP during neuronal differentiation. Shown in d are representative GFP+/TuJ1+ cell images at D5 and D15. Insets, high-powered views of the boxed areas. Expression of GFP mRNA (e) and % of GFP+ cells out of TuJ1 cells (f) at D15 were compared with those at D5. Total numbers of TuJ1+ neurons expressing GFP at D15 (g). *Significantly different from D5 and the cultures transduced with Retro-pLTR at P<0.05 (n=20 microscopic fields). Scale bar, 20 μm. DAPI, 4,6-diamidino-2-phenylindole; GFP, green fluorescence protein.

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