Figure 2
Ectopic overexpression of RBP2 in two RCC cell lines SKRC-39 and SN12C, induced an EMT program. (a) Western blotting assessment of elevated expression of RBP2 protein in RCC cell lines stably transfected with pCDNA-RBP2 in comparison with cells transfected with empty vector alone. (b) Cells with high RBP2 show reduced expression of the epithelial marker E-cadherin and elevated levels of the mesenchymal markers N-cadherin and vimentin. (c) mRNA expression of EMT factors analyzed by RT-PCR. (d) The ChIP assay shows that RBP2 is a DNA binding protein that can directly bind to the promoter sequence of E-cadherin DNA. RT-PCR, PCR with reverse transcription.
