Figure 3
RBP2-nduced EMT-generated stem cell-like cells. (a) FACS analysis shows an increase in CSC markers ALDH, (b) CD133 and (c) CD44 in RBP2-transfected cells. Bar charts indicate the percentage of cells positive for CSC markers (**P<0.01; n=9) (d) quantitative real-time PCR showed high mRNA expression of CSC TFs Sox2, Oct4 and Nanog in RBP2-transfected cells (*P<0.05; **P<0.01; n=3). (e) Western blot shows increased protein levels of Sox2, Oct4 and Nanog in RBP2-transfected cells. (f) Representative microscopy images of tumorsphere formation (× 100) and (g) quantification of tumorspheres formed from three experiments (*P<0.05).
