Figure 4
Exogenous overexpression of RBP2 did not affect cell proliferation but promoted resistance to apoptosis in RCC cell lines SKRC-39 and SN12C. (a) MTT assay shows no change in proliferation rates of RBP2-transfected cells. (b) FACS DNA content analysis for the cell cycle profile of RBP2 expressing cells and control cells. (c) Quantification of apoptotic cells from DNA contents shows a reduced apoptotic population in RBP2-transfected cells. (d) Western blot analysis of proliferating cell nuclear antigen, Bcl-2 and Bax proteins indicating resistance to apoptosis but no change in proliferation of RBP2-transfected cells. (e) ChIP assay indicates binding of RBP2 to the Bcl-2 promoter.
