Figure 4
From: The role of autophagy in allergic inflammation: a new target for severe asthma
The effect of Atg5 knockdown on LC3 expression, AHR, and airway inflammation. (A) LC3 expression after Atg5 knockdown. (B) Changes in lung resistance in response to increasing doses of methacholine were assessed 48 h after the final challenge. (C) Airway inflammatory cell counts in BALF. (D) Cytokine levels in BALF. (E) H&E-stained lung histology. (F) PAS-stained lung histology. (a) PBS/2% OVA-challenged mice. (b) OVA/1% OVA-challenged mice. (c) OVA/2% OVA challenged mice. (d) Quantification of inflammatory cells or PAS-positive cells. BALF, bronchoalveolar lavage fluid; H&E, hematoxylin and eosin; OVA, ovalbumin; PAS, periodic acid-Schiff; PBS, phosphate-buffered saline.
