Figure 4

Involvement of BubR1 in RSK2 depletion induced accumulation of mitotic cells. (a) Metaphase cells stained for ACA, BubR1 and DNA. Ratio of intensity of BubR1 on kinetochores (ACA) was measured from 80 kinetochore pairs per cell. A representative data from three independent experiments are shown. mean±s.d. ***P<0.001 by Student’s t-test (n=10). Scale bar, 5 μm. (b) Inter-kinetochore distance of metaphase cells was measured from >100 kinetochore pairs per cell. Left: a representative data from three independent experiments are shown as mean±s.d. ***P<0.001 by Student’s t-test (n=70). Right: the percentage of the cells having indicated inter-kinetochore distance is shown. A representative data from three independent experiments. (c) Mitotic index was measured at 48 h after the transfection with siRSK2 and/or siBubR1. A representative data from three independent experiments are shown as mean±s.d. ***P<0.001 by Student’s t-test (n=300). (d) Mitotic duration was measured from the time when cells roundup to anaphase onset. A representative data from three independent experiments are shown as mean±s.d. ***P<0.001 by Student’s t-test (n=80).