Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Mitochondrial pyruvate dehydrogenase phosphatase 1 regulates the early differentiation of cardiomyocytes from mouse embryonic stem cells

Figure 1

Cardiomyocyte differentiation from mESCs. (a) Undifferentiated R1 mESCs were dissociated and cultured using the hanging drop method to produce differentiated EBs. EBs were transferred to culture plates after 4 days to promote differentiation into cardiomyocyte-included heterogeneous cells. (b) The self-beating capacity and (c) beating number per minute was then measured. (d) Pluripotency and cardiac-specific proteins were stained with an antibody against Oct 3/4 and cTnT. (e–g) Three types of action potentials (e: nodal, f: atrial, g: ventricular) were recorded in mESC-derived cardiomyocytes by patch clamp. EB, embryoid body.

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