Figure 1
From: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells
Spheroid cells from A2780 ovarian cancer cells have stem cell-like characteristics. (a) Bright-field images of spheroids (SP) generated from adherent A2780 ovarian cancer cells (AD) cultured in spheroid-forming conditions (Neurobasal media) are shown. Images of spheroids on day 10 after plating into spheroid culture condition are shown. Scale bar, 100 μm. (b) Western blotting analysis of adherent A2780 cells and spheroids with the indicated markers is shown. (c) Flow cytometric analysis of adherent A2780 cells and spheroid cells after incubation with ALDH substrate is shown. (d) Time course analysis of cell counts after plating 100 cells from A2780 adherent cells or spheroids per well is shown. *P<0.05. (e) Time course results of an MTT assay with A2780 adherent cells and spheroid cells in the absence or presence of paclitaxel (left panel, 0.1 μM) or doxorubicin (right panel, 1 μM) are shown. *P<0.05.
