Figure 3
From: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells
Altering HMGA1 expression changes the expression of stemness-related gene expression. (a) Western blotting analysis of A2780 spheroid cells with (sh-HMGA1) or without (sh-Control) knockdown of HMGA1 is shown with the indicated probes. (b) Western blotting analysis of HMGA1-overexpressing A2780 cells (HMGA1) or control A2780 cells (Control) is shown with the indicated probes. (c) The results of flow cytometric analysis of A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (upper panel) or HMGA1-overexpressing A2780 adherent cells in comparison with control A2780 adherent cells (lower panel) after incubation with ALDH substrate are shown.
