Figure 5
From: Crucial role of HMGA1 in the self-renewal and drug resistance of ovarian cancer stem cells
HMGA1 regulates ABCG2 promoter activity and drug resistance. (a) Schematic representations of the −1285 and −628 5′ upstream regions of the ABCG2 promoter are shown. HMGA1-binding sites are marked by boxes. (b) The results of a transcriptional activity assay after co-transfecting an ABCG2 promoter construct (−1285 or −628) with an HMGA1-overexpression construct into A2780 cells are shown (upper panel). *P<0.05. The results of western blotting analysis are shown in the lower panel. (c) The results of a transcriptional activity assay after co-transfecting an ABCG2 promoter construct (−1285, ΔHMGA1-1, ΔHMGA1-2 or ΔHMGA1-3) with an HMGA1-overexpression construct into A2780 cells are shown (upper panel). *P<0.05. The results of western blotting analysis are shown in the lower panel. (d) The results from a time course MTT assay with A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (left panel), or with HMGA1-overexpressing A280 cells in comparison with control A2780 adherent cells (right panel) in the presence of paclitaxel (0.1 μM), are shown. *P<0.05. (e) The results from a time course MTT assay with A2780 spheroid cells with HMGA1 knockdown in comparison with control A2780 spheroid cells (left panel), or with HMGA1-overexpressing A280 adherent cells in comparison with control A2780 adherent cells (right panel) in the presence of doxorubicin (1 μM), are shown. *P<0.05.
