Figure 2 | Experimental & Molecular Medicine

Figure 2

From: MSP is a negative regulator of inflammation and lipogenesis in ex vivo models of non-alcoholic steatohepatitis

Figure 2

MSP ameliorates LPS-induced inflammation in primary mouse hepatocytes. (a) Representative western blot (top) and quantification of the fold change relative to the vehicle control (bottom) of AMPK and ACC phosphorylation for the indicated treatments. Data are expressed as the mean±s.e.m. (n=6). *P<0.05 vs Ctl; #P<0.05 vs LPS. (b–d) Real-time qPCR analysis of genes involved in inflammation (b), lipogenesis (c), and fatty acid oxidation (d) from the hepatocytes treated as indicated. Each value indicates the amount of mRNA relative to the vehicle control-treated hepatocytes. Cyclophilin A was used as the invariant control. Data are expressed as the mean±s.e.m. (n=6). *P<0.01 vs Ctl; #P<0.05 vs LPS; &P<0.05 vs LPS±MSP or LPS±AICAR. ACC, acetyl CoA carboxylase; AMPK, AMP-activated protein kinase; Ctl, control; LPS, lipopolysaccharide; MSP, macrophage-stimulating protein; qPCR, quantitative PCR.

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