Figure 4

RON receptor relays the downstream effects of MSP in HepG2 cells. (a) Representative western blot of shRNA-induced knockdown of RON (top) and quantification of the fold change relative to shScr (bottom). Data are expressed as the mean±s.e.m. of three independent experiments. *P<0.01 vs shScr. (b) Representative western blot (left) and quantification of the fold change relative to Ctl (bottom) of AMPK and ACC phosphorylation in cells challenged with PA, in the absence or presence of MSP, in RON knockdown cells. Data are expressed as the mean±s.e.m. of three independent experiments. *P<0.01 vs Ctl; ^#P<0.05 vs shScr±PA±MSP. (c) Real-time qPCR analysis of genes involved in inflammation (TNFα, IL-6) and lipogenesis (FAS, SREBP-1c) in cells challenged with PA, in the absence or presence of MSP, in RON knockdown cells. Values are expressed as the mean±s.e.m. of three independent experiments. *P<0.01 vs Ctl; **P<0.05 vs shScr+PA; ^#P<0.05 vs shScr+PA+MSP. ACC, acetyl CoA carboxylase; AMPK, AMP-activated protein kinase; Ctl, control; MSP, macrophage-stimulating protein; PA, palmitic acid; qPCR, quantitative PCR; RON, recepteur d'origine nantais.