Figure 5 | Experimental & Molecular Medicine

Figure 5

From: MSP is a negative regulator of inflammation and lipogenesis in ex vivo models of non-alcoholic steatohepatitis

Figure 5

MSP exerts anti-inflammatory effects in BMDMs. (a) Representative western blot (left) and quantification of the fold change relative to vehicle control (right) of RON, AMPK and ACC phosphorylation (b) ELISA analysis of TNFα and IL-10 production, and (c) Gene expression of pro-inflammatory cytokines Tnfα, Il-6, Mcp-1 and orphan nuclear receptor Shp in oxLDL+LPS challenged BMDMs, in the absence or presence of MSP. Values are expressed as the mean±s.e.m., n=5 per group. *P<0.01 vs Ctl; **P<0.05 vs oxLDL+LPS. ACC, acetyl CoA carboxylase; AMPK, AMP-activated protein kinase; BMDM, bone marrow-derived macrophage; Ctl, control; IL-10, interleukin 10; LPS, lipopolysaccharide; Mcp-1, monocyte chemoattractant protein-1; MSP, macrophage-stimulating protein; oxLDL, oxidized low-density lipoprotein; RON, recepteur d'origine nantais; TNFα, tumor necrosis factor-α.

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