Figure 3
From: CTHRC1 promotes angiogenesis by recruiting Tie2-expressing monocytes to pancreatic tumors
CTRHC1 enhances infiltration of TEMs into pancreatic cancer tissues. (a) CTHRC1-overexpressing or mock-transfected MiaPaCa-2 cells (left panel) and 105 shCon- or shCTHRC1-expressing Panc-1 cells (right panel) were orthotopically injected into nude mice (n=5). Four weeks after tumor challenge, the percentage of infiltrated TEMs was determined by flow cytometry analysis of cells immunostained for Tie2 and F4/80. Data are reported as the mean±s.d. (*P<0.01, #P<0.05, **P<0.001 versus shCon or mock-transfected cells). (b) Matrigel containing PBS (control) or rCTHRC1 (1 or 5 μg ml−1) was injected into C57BL/6 mice (n=5). Seven days after injection, Matrigel plugs were excised and stained for infiltrating TEMs using anti-F4/80 and anti-Tie2 antibodies. TEMs were quantified by counting the number of F4/80 and Tie2 double-positive cells per field for each Matrigel plug. Data are reported as the mean±s.d. (**P<0.001 versus vehicle). CTHRC1, collagen triple-helix repeat-containing 1; PBS, phosphate-buffered saline; TEMs, Tie2-expressing monocytes.
