Figure 5
PMCA generates α-synuclein aggregates from in vivo samples. (a) Immunoblotting of Thy1-A53T mice brain homogenate (seed A) induced sPMCA products with the indicated cycles. (b) Immunoblotting of mPrP-A53T spinal cord homogenates (seed B) induced sPMCA products with indicated cycles. (c) PK digestion patterns of PMCA products with different tissue seeds. Note the extra band (black arrow) on seed B-derived PMCA products. (d) CD spectra revealed seeded aggregates with different PMCA products and seeds possessing a β-sheet rich structure. (e) PK digestion patterns of seeded aggregates with different PMCA seeds. Seeded aggregates with different seeds exhibited similar PK resistant fragments with their respective seeds.
