Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Cadmium-induced ER stress and inflammation are mediated through C/EBP–DDIT3 signaling in human bronchial epithelial cells

Figure 1

Effect of cadmium (Cd) on cell viability and pro-inflammatory cytokine expression. BEAS-2B cells were treated with Cd (1 to 100 μM) in medium containing antibiotics and incubated for 24 or 48 h. Cell viability was measured by the MTS assay. Triton X-100 (0.1%, Triton), as suggested by the manufacturer, leads to complete cell death, and so the Triton-treated group was set as 0% of the survival rate and non-treated group sets as 100% (a). Transcriptional levels of pro-inflammatory cytokines (IL-1α, IL-1β, IL-6, IL-8, TNFα, MMP9, COX2 and iNOS) were analyzed using SYBR green-based quantitative real-time PCR (b). Supernatant IL-6 levels were measured by enzyme-linked immunosorbent assay (ELISA) (c). Protein levels of pro-inflammatory cytokine (IL-1β, TNFα, IL-6, iNOS or COX2) were detected by immunoblotting (d). All data shown are representative of three biological replicates. *P<0.05; **P<0.01.

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