Figure 7
From: IL-33 promotes IL-10 production in macrophages: a role for IL-33 in macrophage foam cell formation
Identification of transcription and target sequences that react with IL-33. (a) Effects of IL-33 on intra-nuclear Sp1 expression as well as STAT3 and STAT4 phosphorylation. (b) Bioinformatic analysis of STAT3-binding sites within the −2000 to −1752 bp of the 5′-flank of the IL-10 transcription start site. (c) Homology analysis of the predicted binding STAT3 site among five mammalian species. (d) Effects of TTC base deletion by site-directed mutagenesis on promoter activity indicated by luciferase activity. (e, f) Effects of a STAT3 inhibitor, cryptotanshinone, on IL-33-induced IL-10 mRNA (e) and protein (f) production. (g) CHIP analysis using a STAT3 antibody and primers specific to IL-10 promoter segments following PBS or IL-33 (30 ng ml−1) treatment. p-STAT3, phosphorylated STAT3; p-STAT4, phosphorylated STAT4; t-STAT3, total STAT3; t-STAT4, total STAT4; wild type, pGL3-Basic-IL10.1-Wild type; Mutant, pGL3-Basic-IL10.1-Mutant; IL-33-P1, qPCR using primer set 1; IL-33-P4, qPCR using primer set 6 (all primer sets are listed in Supplementary Materials). *P<0.05 versus PBS treatment; #P<0.05 versus IL-33-P1. The data in a and d–g represent three independent experiments.
