Figure 1
From: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target
Expression of NGF, proNGF and their cognate receptors, TrkA and p75NTR, in human livers with hepatolithiasis. (a) Hepatolithiasis samples (n=5) included lithiasis and contralateral liver tissues. Paralesional protein extracts from patients with hepatocellular carcinoma were the non-hepatolithiasis controls (NHC, n=4) and were subjected to western blotting detection of NGF, proNGF, TrkA and p75NTR levels. The scan density data of NHC and hepatolithiasis groups are shown in Supplementary Tables S3 and S4, respectively. Densitometrical analyses of the expression levels of NGF and proNGF (b), as well as those of TrkA and p75NTR (c), are shown as the mean±s.e.m. of their ratios to internal actin controls. *P<0.05 vs NHC levels, #P<0.05 vs contralateral levels. (d) RT-qPCR detection of NGF, TrkA and p75NTR mRNA levels in human livers with hepatolithiasis. Spearman’s correlation analysis of the relationships of hepatic NGF (e) and proNGF expression (f) with serum total bilirubin levels.
