Figure 6
From: Nerve growth factor upregulates sirtuin 1 expression in cholestasis: a potential therapeutic target
Effects of simultaneous administration with NGF and its receptor blockers on bile duct ligation (BDL)-induced apoptosis of parenchymal hepatocytes. The ICR mice receiving BDL surgery were intraperitoneally administered recombinant NGF (1 mg kg−1; n=3) twice weekly and simultaneous daily injections of PBS (n=6), GW441756 (GW; 1 mg kg−1; n=6), DMSO (n=6) or PD90780 (PD; 1 mg kg−1; n=6). After 2 weeks of treatment, pooled liver extracts of each group were subjected to western blotting to detect both propeptides and cleaved forms of caspase-3 and PARP (a). The activation of caspase-3 (b) and PARP (c) was densitometrically analyzed and is shown as the cleavage-to-propeptide ratios and normalized to negative control (NC) levels. (d) Representative images of IHC staining for activated PARP in the mouse livers. (e) Quantitative measurement of PARP activation as shown by labeling index. (f) Representative microphotographs of TUNEL staining showing the apoptotic events in mouse liver sections. Scale bars=50 μm. (g) Analysis of TUNEL-positive parenchymal cells indicated that GW treatment significantly increased apoptotic cell death in BDL-induced cholestatic injured livers. All data are shown as the mean±s.e.m. *P<0.05 between indicated groups. ND, not detectable. NS, not significant.
