Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Proangiogenic functions of an RGD-SLAY-containing osteopontin icosamer peptide in HUVECs and in the postischemic brain

Figure 3

Induction of tube formation by 20-amino-acid OPN peptide (OPNpt20) in human umbilical vein endothelial cell (HUVEC) cultures. OPNpt20 (1 μM)-induced tube formation was examined in HUVECs treated with OPNpt20 (1 μM) or OPNpt20-Db (mutant OPN peptide with both RGD and SLAY replaced; 1 μM) for 12 h in the presence or absence of anti-αvβ3 antibody or immunoglobulin G (IgG). Representative images obtained from Image J analyzer (National Institute of Health, Bethesda, MD, USA) are presented (green, branches; yellow, master segments; blue, tubes; red, master junctions) (a). Tube numbers were counted (b), and tube lengths were measured (c). The results are presented as the mean±s.e.m. (n=3). Scale bars, 500 μm. *P<0.05, **P<0.01 between the indicated groups.

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