Figure 4

PIK3R3 regulates the expression of PPARα. (a) Quantitative PCR (upper panel) analysis and western blot (bottom panel) analysis showing the expression level changes of Pparα in mice livers during HFD feeding. Data are expressed as the means±s.e.m. *P<0.05; **P<0.01. (b) Immunohistochemistry analysis showing the expression level changes of hepatic Pparα in the livers of the mice in Figure 2c. (c) Western blot analysis showing the expression level changes of hepatic Pparα in the livers of the mice in Figure 2c. (d) Immunohistochemistry analysis showing the expression level changes of hepatic Pparα in the livers of the mice in Figure 3c. (e) Western blot analysis showing the expression level changes of hepatic Pparα in the livers of the mice in Figure 3c. (f) PIK3R3 was knocked down in HepG2 and LO2 cells by siRNAs transfection. Western blot analysis showing the expression levels of PPARA and PPARG. (g) PIK3R3 was overexpressed in hepatic HepG2 and LO2 cells by plasmid transfection. Western blot analysis showing the expression levels of PPARA and PPARG.