Figure 6
From: PIK3R3 regulates PPARα expression to stimulate fatty acid β-oxidation and decrease hepatosteatosis
PIK3R3 regulation of PPARα expression was dependent on HNF4α. (a) PIK3R3 was overexpressed in LO2 cells and knocked down in HepG2 cells; western blots were used to detect the protein levels of PIK3R3 and HNF4α. (b) PIK3R3 was overexpressed in LO2 cells with or without HNF4α knockdown; western blot analysis showing the expression levels of PIK3R3, HNF4α and PPARα. (c) PIK3R3 was knocked down in LO2 cells with or without HNF4α overexpression; western blot analysis showing the expression levels of PIK3R3, HNF4α and PPARα. (d) ChIP assay and quantitative PCR analysis showing the binding activity of HNF4α to the PPARα promoter after the overexpression of PIK3R3. (e) ChIP assay and quantitative PCR analysis showing the binding activity of HNF4α to the PPARα promoter after the downregulation of PIK3R3. Data are expressed as the means±s.e.m. *P<0.05.
