Figure 4

Fascin is important for Rab25-induced cancer invasion. (a) Cells were transfected with indicated vectors. Quantitative RT-PCR (mean±s.d. *P<0.05, **P<0.01 and ***P<0.001 versus control vector). (b) SKOV-3 cells were co-transfected with indicated vectors. Luciferase activity (mean±s.d. **P<0.01 versus Rab25 with Fascin promoter overexpression). (c, d) MCF-7 cells were co-transfected with indicated vectors and siRNAs. Immunoblotting. (e) MCF-7 cells were stimulated with VEGF_165aa (50 ng ml⁻¹) for 1 h. Immunoblotting. (f) MCF-7 cells were transfected with indicated siRNAs, serum starved and stimulated with VEGF_165aa (50 ng ml⁻¹) for 1 h. Immunoblotting. (g) MCF-7 cells were co-transfected indicated vectors and siRNAs. Invasion assay (mean±s.d. ***P<0.001 versus control vector with scrambled siRNA, ^###P<0.001 versus Rab25 overexpression with scrambled siRNA). (h) SKOV-3 cells were transfected with indicated siRNAs, serum-starved and treated with VEGF_165aa (50 ng ml⁻¹). Invasion assay (mean±SD ***P<0.001 versus scrambled siRNA, ^###P<0.001 versus scrambled siRNA with VEGF_165aa) (i) MCF-7 cells transfected with indicated vectors were treated with VEGF neutralizing antibody (VEGF ab, 5 μg ml⁻¹, 24 h). Immunoblotting. Representative results are presented from at least three independent experiments with similar results.