Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Arginase II inhibition prevents interleukin-8 production through regulation of p38 MAPK phosphorylation activated by loss of mitochondrial membrane potential in nLDL-stimulated hAoSMCs

Figure 1

The arginase II isoform is dominantly expressed and targeted to mitochondria in human aortic smooth muscle cells. (a) Reverse transcription–PCR (RT-PCR) was performed with isoform-specific primers for arginase I and II after native low-density lipoprotein (nLDL) stimulation at different time intervals. (b) Western blotting analysis was performed with specific anti-sera against arginase I and II in cell lysates that were either treated with nLDL or left untreated. L, liver lysate for arginase I, K, kidney lysate for arginase II. (c) Cells were stained with the mitochondrial-specific dye MitoTracker-Red and costained with anti-arginase II antibody (green). The scale bar indicates 50 μm. (d) The cell lysates were fractionated into cytosolic and mitochondrial fractions, and voltage-dependent anion channel (VDAC) and β-tubulin were used as mitochondrial and cytosolic marker proteins, respectively. Arginase II was identified in the mitochondrial fraction (M) but not in the cytosolic fraction (c) in human aortic smooth muscle cells (hAoSMCs; n=3) *P<0.01.

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