Figure 1 | Experimental & Molecular Medicine

Figure 1

From: Protection of nigral dopaminergic neurons by AAV1 transduction with Rheb(S16H) against neurotoxic inflammation in vivo

Figure 1

Transduction of nigral DA neurons with AAV1-GFP and AAV1-Rheb(S16H) in normal adult mice. (a) The expression of GFP and FLAG (brown reaction product) in the SN at 3 weeks after AAV1-GFP and AAV1-Rheb(S16H) administration for each viral injection. No expression of FLAG was observed in the non-injected control side (CON). The area inside the dotted lines is the substantia nigra pars compacta. Scale bar, 200 μm. (b) Double-immunofluorescence staining for TH (red) and GFP (green) and TH and FLAG (green) in the SN. Arrow heads indicate increased neuronal size induced by Rheb(S16H) expression in the nigral DA neurons. Scale bar, 20 μm. (c) Double-immunofluorescence staining for GFAP/Iba1 (red) and GFP (green), and GFAP/Iba1 and FLAG (green) in the SN. Scale bar, 20 μm.

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