Figure 3 | Experimental & Molecular Medicine

Figure 3

From: PI3Kδ contributes to ER stress-associated asthma through ER-redox disturbances: the involvement of the RIDD–RIG-I–NF-κB axis

Figure 3

IC87114 attenuates stress-associated reactive oxygen species (ROS) accumulation in the endoplasmic reticulum (ER) of lung epithelial cells. (a) The 4-hydroxy-2-nonenal (4-HNE) and dihydroethidine hydrochloride (DHE) staining in lung tissues obtained from ovalbumin/lipopolysaccharide (OVA/LPS)-treated mice, saline (SAL)-treated mice and OVA/LPS-treated mice administered 1 mg kg−1 IC87114. (b) Lysates from lung tissues analyzed for the presence of oxidized proteins by oxyblot analysis. 4-HNE and malondialdehyde (MDA) (c) and hydrogen peroxide levels (d) in lung ER fractions from IC87114-treated mice. (e) GSH/GSSG ratio in the lung ER fraction. In (d) and (e), values are presented as the mean (±s.e.m.) fold changes relative to the control (SAL) group (n=6 animals per experimental group). (f) Immunoblotting was performed using an anti-calnexin antibody as an ER marker and an anti-voltage-dependent anion-selective channel (anti-VDAC) antibody as a mitochondrial marker, after isolating ER fractions. Mice challenged with saline (SAL) were used as the control group. #P<0.05 versus SAL; *P<0.05 versus OVA/LPS only.

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