Figure 3 | Experimental & Molecular Medicine

Figure 3

From: Nucleophosmin modulates the alleviation of atopic dermatitis caused by the marine-derived compound dihydroaustrasulfone alcohol

Figure 3

Effects of WA-25 on NF-κB activity and NF-κB–NPM interaction after 6 h. (a) Western blot and ratio of p65 NF-κB translocation is shown. (b) Western blot analysis of sirt-6. Relative densities of sirt-6 protein expression are provided. (c) Western blot analysis of NPM. Relative densities of nuclear and total NPM protein expression are provided. (d) p65 NF-κB (green) and NPM (red) immunostaining. Scale bar=5 μm. (e) Immunostaining of LAMP-1 (green) and NPM (red). Scale bar=7.5 μm. (f) Immunoprecipitation of NPM and immunoblot analysis of p65 NF-κB. Relative densities of NPM and p65 NF-κB are provided. *Significantly (P<0.05) different from the LPS-stimulated group; n=6 per group.

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