Figure 1

The genomic location and bioinformatics analyses of uc.4. (a) Bioinformatics analyses of uc.4 using the UCSC genome browser (http://genome.ucsc.edu). (b) Conservation of uc.4 between chimp, mm3, rat and chicken. Chimp, chimpanzee; mm3, mouse. (c) The results sequencing that was performed to verify the conservation of uc.4. (d) uc.4 expression levels were significantly increased in human fetal VSD heart tissues. *P<0.05 versus controls, Student’s t-test. (e) The profile of uc.4 expression in six organs (heart, liver, spleen, lung, kidney and brain). The expression level of uc.4 was higher in lung tissue than other tissues. (f) qRT-PCR analyses of uc.4 in cytoplasmic and nuclear fractions of P19 cells. 45S rRNA and mitochondrial 12S rRNA served as positive controls. ***P<0.001, Student’s t-test. There were three replicates for each experiment. Data are presented as the means±s.d. qRT-PCR, quantitative real-time polymerase chain reaction; VSD, ventricular septal defect.